Knox Trail is a historic road that runs through the town of Acton, Massachusetts. It was part of the route that General Henry Knox used to transport 59 cannons from Fort Ticonderoga to Boston during the American Revolutionary War. The trail is marked by granite monuments that commemorate the historic event. Knox Trail is a popular destination for history buffs, hikers, and bikers.

Knox Trail goes north from Main Street and is parallel to the Assabet River.