Stretching from Afton to Janesville, the Peace Trail offers a paved, tree-shaded route paralleling the Rock River and an active rail line. From its northern end in Janesville, the trail crosses the Rock River via a protected pedestrian walkway on the S Willard Avenue bridge and then meanders through Rockport Park (2801 Rockport Rd). The park is a picturesque open space offering trails, a pool, concession stands, restrooms, a historic farmstead, and the Peace Park Playground. 

From Rockport Park in Janesville, the Peace Trail follows the river south through Afton to the northern outskirts of Beloit, where it enters Big Hill Park. Nestled along the west bank of the Rock River, the heavily wooded 197-acre park offers hiking trails, an environmental center, picnic areas, and playgrounds.

At the trail's northern end by Rockport Park, it connects with the Rock River Parkway Trail, which continues northeast another 2.5 miles along the river and links with the Kiwanis Bike Trail and the Spring Brook Trail.

Trailhead parking is available adjacent to the Janesville Wastewater Plant at the end of Tripp Road. At its northern end, the Fisher Creek parking lot is available near the intersection of County Highway D (Afton Road) and W. Rockport Park Drive. Parking is also available in Rockport Park (2801 Rockport Road).

In Beloit, parking is available in Big Hill Park.